| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Venema, Nicole E (19)
|
VATIDE |
50.33 |
53.36 |
3.03 |
| 2 |
Secrest, Jennifer L (19)
|
VATIDE |
51.06 |
53.48 |
2.42 |
| 3 |
Durak, Anna J (20)
|
VATIDE |
51.26 |
53.62 |
2.36 |
| 4 |
Knapp, Sophia K (16)
|
VATIDE |
52.35 |
56.15 |
3.80 |
| 5 |
Swatts, Chloe E (15)
|
VATIDE |
55.13 |
57.89 |
2.76 |
| 6 |
Tran, Emilie H (15)
|
VATIDE |
57.52 |
58.32 |
0.80 |
| 7 |
Franco, Lucia J (15)
|
VATIDE |
58.12 |
58.35 |
0.23 |
| 8 |
Claesson, Sarah M (16)
|
VATIDE |
55.45 |
58.50 |
3.05 |
| 9 |
Schooneman, Kim (16)
|
VATIDE |
56.00 |
59.66 |
3.66 |
| 10 |
Flora, Erin N (17)
|
VATIDE |
56.82 |
59.87 |
3.05 |
| 11 |
Stedje-Larsen, Anna B (16)
|
VATIDE |
58.50 |
1:00.09 |
1.59 |
| 12 |
Benson, Alaiyah M (15)
|
VATIDE |
1:01.42 |
1:00.33 |
-1.09 |
| 13 |
Worth, Spencer R (16)
|
VATIDE |
57.46 |
1:00.97 |
3.51 |
| 14 |
Moore, Caitlyn D (16)
|
VATIDE |
57.50 |
1:02.81 |
5.31 |
| 15 |
Roberto, Sophia R (15)
|
VATIDE |
1:00.74 |
1:03.19 |
2.45 |
| 16 |
Neff, Megan M (17)
|
VATIDE |
|
1:05.14 |
- |
| 17 |
Saddler, Haylee T (16)
|
VATIDE |
1:02.86 |
1:09.53 |
6.67 |
| 18 |
Yeiter, Adriana G (16)
|
VATIDE |
|
1:11.76 |
- |
| 19 |
Andersen, Victoria J (16)
|
VATIDE |
|
1:14.72 |
- |
| 20 |
Hudspeth, Lauren C (17)
|
VATIDE |
1:24.35 |
1:30.80 |
6.45 |
|
Tabor, Trista J (15)
|
VATIDE |
|
NS |
- |